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planation that Lemna rises to th --------=_MB71654DB8-2E04-493B-B3A4-647F7308BA4F Content-Type: text/plain; format=flowed; charset=utf-8 Content-Transfer-Encoding: quoted-printable Hi Steve & All, Yes we can agree with the fact that they sink in the fall and rise=20 in the spring and, by analogy with plant processes in general one may=20 assume that these changes in position are under hormonal control. The=20 long rambling discussion last year was largely an attempt to consider=20 possible mechanisms. One may take it as given that photosynthesis=20 requires light and respiration continues under all conditions but slows=20 as temperature decreases. Also given; CO2 is more soluble in water than =20 O2. Note that my control was the dish on the windowsill and treated was=20 the dish in darkness. The original article proposed starch accumulation in the fall as the= =20 mechanism of sinking and loss in the spring as the mechanism of rise.=20 Someone last year proposed an alternative; The alternative being-- With shortening days in the fall and=20 consequently less photosynthesis, the cellular release of CO2 would=20 exceed the release of O2. And as photosynthesis approached zero, CO2=20 would predominate in the gas filled spaces. This reversal in gas=20 composition ( O2 > CO2) would render gas filled spaces, which were not=20 isolated from the medium, more subject to flooding due to the greater=20 solubility of CO2. If the above reasoning had been valid then my treated plants, kept=20 in darkness for nearly a week, would have all sunk. They showed no=20 greater tendency to sink than the control. Consequently flooding of=20 intercellular spaces (or specialized aerenchyma) by water is not the=20 mechanism by which average density of Lemna increases in the fall. So I conclude that accumulation of starch (density 1.5) in the fall,= =20 as growth slows to zero and respiration slows as temperature lowers, is=20 the mechanism of sinking. And conversely greater respiration, due to=20 rising temperature and resumption of growth leads to loss of starch, a=20 decrease in average density and rise to the surface. The original article noted that Lemna is more efficient than corn as= =20 a producer of starch; impressive. I suspect the starch content in the=20 fall would be higher than in the spring and this I think is true of most=20 plans in temperate climates. Yt, DW Kentville ------ Original Message ------ From: "Stephen Shaw" <srshaw@Dal.Ca> To: "naturens@chebucto.ns.ca" <naturens@chebucto.ns.ca> Sent: 9/28/2018 11:57:50 PM Subject: Re: [NatureNS] Duckweed >Hi Dave, >I=E2=80=99d forgotten about this, but isn=E2=80=99t the primary observatio= n to explain=20 >that the plants routinely sink in the winter and rise again in the=20 >spring? What the mechanism is, is an interesting but secondary=20 >consideration. In your =E2=80=98experimental=E2=80=99 case with lighted= duckweed, it=20 >should be producing and accumulating some sort of gas mixture by=20 >September's photosynthesis, maybe plus some concurrent respiration. =20 >Your =E2=80=98control', dark duckweed, should have no photosythesic activi= ty,=20 >so only respiration should produce any gas at all, presumably CO2. > >The problem is that neither =E2=80=98expt' nor =E2=80=98control' actually= sank on Sept=20 >27. Since the plant material including any dense starch should anyway=20 >have an average density >1g/cc (i.e. greater than plain water*),=20 >excluding any gas spaces, both cases must still be floating according=20 >to some undissolved gas inclusions, though not necessarily the same mix=20 >of CO2/O2 in the two cases. You get an A+ for industry and design, but=20 >Lemna gets an F for cooperation. > >*Something I=E2=80=99d not considered that you may know about but I don=E2= =80=99t is=20 >oil/fat production in plants, which obviously is important for some=20 >land plants (canola, sesame, olives etc). Oils have specific gravities=20 ><1, around 0.9 g/cc, so enough oil volume could be sufficient to float=20 >a water plant without invoking gas spaces. If something dense like=20 >starch gets converted to an oil in spring, could that contribute, or is=20 >this just fantasy? > >Is it possible that the trigger for sinking, whatever its mechanism, is=20 >a particular drop in day length that had not yet been closely=20 >approached by Sept 27, and especially in your toasty living room=20 >(temperature might be another factor)? If you haven=E2=80=99t flushed th= em in=20 >frustration, you should convey both your test subjects to an unheated=20 >outhouse and extend your experiment there at least to freeze-up. >Steve >--------------------------------------------------------- >On Sep 27, 2018, at 12:35 PM, David <dwebster@glinx.com> wrote: > >>Dear All, >> I have had my eye open this summer for some Lemna to test the gas=20 >>composition idea; the more soluble CO2 predominating as days shorten=20 >>leading to flooding of gas spaces and sinking; photosynthesis=20 >>predominating in spring as days lengthen leading to flooded air spaces=20 >>being filled by less soluble O2 and rise to the surface. >> And I was able to collect some on Sept 21 without having to wade=20 >>in muck of uncertain depth. So I set up a simple trial; some kept in=20 >>darkness, except for a few minutes daily for observation, and some=20 >>kept on a windowsill. >> By Sept 27 those kept in darkness showed no tendency to sink but=20 >>were noticeably less green than those exposed to daylight and some=20 >>artificial light in the evening. This leads to the conclusion that=20 >>flooding of intercellular gas spaces when CO2 predominates does not=20 >>cause sinking of Lemna. The converse explanation that Lemna rises to=20 >>the surface in the spring when intercellular spaces become filled by=20 >>less soluble O2 is accordingly voided. >> The explanation given in the link posted by Nancy on Oct 3, 2017=20 >>is consequently more sound. Starch accumulation in the fall. as=20 >>photosynthesis exceeds respiration at lowering temperature, leads to=20 >>sinking. Starch consumption, as temperature warm in the spring and new=20 >>buds start to grow, leads to rise to the water surface. [density of=20 >>starch is high; 1.5 g/cm^3] >> >>Yt, DW, Kentville >> >> >> >>------ Original Message ------ >>From: "Stephen Shaw" <srshaw@Dal.Ca> >>To: "naturens@chebucto.ns.ca" <naturens@chebucto.ns.ca> >>Sent: 3/10/2018 3:20:47 PM >>Subject: Re: [NatureNS] Duckweed >> >>>Interesting puzzle, perhaps not so simple. Any cell system like a=20 >>>live leaf is bound to be somewhat denser than pond water, regardless=20 >>>of denser starch, so will naturally sink at all times unless kept=20 >>>buoyant by the extracellular gas bubbles or some other modification. =20 >>>What seems to need explaining is why the bubbles are maintained in=20 >>>summer (dissolved gases can pass through lipid cell membranes=20 >>>quickly, so you=E2=80=99d expect them to dissolve out into the surroundi= ng=20 >>>pond), and why they disappear in winter. He doesn=E2=80=99t seem to kno= w if=20 >>>it=E2=80=99s CO2 or O2 in the bubbles, or both. Perhaps they are largel= y O2=20 >>>in summer and dissolve and normally pass out of the leaf to oxygenate=20 >>>the pond and dissolved CO2 moves in, but leaf photosynthesis is=20 >>>sufficiently high in summer to maintain them, despite these losses. =20 >>>In winter gas production simply stops, and the non-buoyant plants=20 >>>sink. As winter ends, respiration produces bubbles with a different=20 >>>gas, CO2, and they rise again then switch over to O2 as=20 >>>photosynthesis picks up. >>> >>>It therefore seems likely that these leaves have some special surface=20 >>>coating that slows down gas exchange with the pond. Maybe they still=20 >>>retain stomata under the leaf which normally facilitate gas exchange=20 >>>for leaves in air, but these are modified to block or regulate gas=20 >>>exchange in water? Maybe this is already known, just not to us here? >>>Steve >>> >>>On Mar 10, 2018, at 10:45 AM, David <dwebster@glinx.com> wrote: >>> >>>>Hi Nancy & All, >>>> I admit not having wondered about this but I think he makes a=20 >>>>simple process complicated. The air pockets to which he refers are=20 >>>>presumably intercellular spaces which in the absence of=20 >>>>photosynthesis presumably can become water filled. Starch has a=20 >>>>specific gravity of 1.5 g/mL and, in cooler weather starch will=20 >>>>accumulate when respiration slows more than photosynthesis and the=20 >>>>submarine will sink. With warming, growth resumes, respiration and=20 >>>>starch consumption rates increase and the submarine rises. >>>>Yt, DW, Kentville >>>> >>>>------ Original Message ------ >>>>From: "nancy dowd" <nancypdowd@gmail.com> >>>>To: naturens@chebucto.ns.ca >>>>Sent: 3/10/2018 9:08:02 AM >>>>Subject: [NatureNS] Duckweed >>>> >>>>>This is an interesting article on Duckweed seasonal disappearance=20 >>>>>and reappearance in the Spring 2018 issue of Northern Woodlands=20 >>>>>Magazine.https://northernwoodlands.org/outside_story/article/duckweed-= migration >>>>> >>>>>I had never really thought about where it went in Fall or how it=20 >>>>>re-emerges in Spring. Such an important floating pond plant in=20 >>>>>productive fresh water >>>>> >>>>>Nancy D >>>>> >>>>>Sent from my iPad >>> > --------=_MB71654DB8-2E04-493B-B3A4-647F7308BA4F Content-Type: text/html; charset=utf-8 Content-Transfer-Encoding: quoted-printable <html><head> <style id=3D"css_styles" type=3D"text/css"><!--blockquote.cite { margin-lef= t: 5px; margin-right: 0px; padding-left: 10px; padding-right:0px; border-le= ft: 1px solid #cccccc } blockquote.cite2 {margin-left: 5px; margin-right: 0px; padding-left: 10px;= padding-right:0px; border-left: 1px solid #cccccc; margin-top: 3px; padding= -top: 0px; } a img { border: 0px; } li[style=3D'text-align: center;'], li[style=3D'text-align: right;'] { list= -style-position: inside;} body { font-family: Segoe UI; font-size: 12pt; }--></style></head> <body><div>Hi Steve & All,</div><div>=C2=A0 =C2=A0 Yes we can agree wit= h the fact that they sink in the fall and rise in the spring and, by analog= y with plant processes in general one may assume that these changes in posi= tion are under hormonal control. The long rambling discussion last year was = largely an attempt to consider possible mechanisms. One may take it as giv= en that photosynthesis requires light and respiration continues under all c= onditions but slows as temperature decreases. Also given; CO2 is more solub= le in water than =C2=A0O2.</div><div>=C2=A0 =C2=A0 Note that my control was = the dish on the windowsill and treated was the dish in darkness.</div><div= >=C2=A0 =C2=A0 The original article proposed starch accumulation in the fal= l as the mechanism of sinking and loss in the spring as the mechanism of ri= se. Someone last year proposed an alternative;</div><div>=C2=A0 =C2=A0 =C2= =A0The alternative being-- With shortening days in the fall and consequentl= y less photosynthesis, the cellular release of CO2 would exceed the release = of O2. And as photosynthesis approached zero, CO2 would predominate in the = gas filled spaces. This reversal in gas composition ( O2 > CO2) would r= ender gas filled spaces, which were not isolated from the medium, more subj= ect to flooding due to the greater solubility of CO2.</div><div>=C2=A0 =C2= =A0 If the above reasoning had been valid then my treated plants, kept in d= arkness for nearly a week, would have all sunk. They showed no greater tend= ency to sink than the control. Consequently flooding of intercellular space= s (or specialized aerenchyma) by water is not the mechanism by which averag= e density of Lemna increases in the fall.</div><div>=C2=A0 =C2=A0 So I conc= lude that accumulation of starch (density 1.5) in the fall, as growth slows = to zero and respiration slows as temperature lowers, is the mechanism of s= inking. And conversely greater respiration, due to rising temperature and r= esumption of growth leads to loss of starch, a decrease in average density= and rise to the surface.</div><div>=C2=A0 =C2=A0 The original article noted = that Lemna is more efficient than corn as a producer of starch; impressive= . I suspect the starch content in the fall would be higher than in the spri= ng and this I think is true of most plans in temperate climates.=C2=A0</div= ><div>Yt, DW Kentville=C2=A0</div> <div><br /></div> <div>------ Original Message ------</div> <div>From: "Stephen Shaw" <<a href=3D"mailto:srshaw@Dal.Ca">srshaw@Dal.C= a</a>></div> <div>To: "naturens@chebucto.ns.ca" <<a href=3D"mailto:naturens@chebucto.= ns.ca">naturens@chebucto.ns.ca</a>></div> <div>Sent: 9/28/2018 11:57:50 PM</div> <div>Subject: Re: [NatureNS] Duckweed</div><div><br /></div> <div id=3D"xc5dd5f1fc8d549c" style=3D"word-wrap: break-word; -webkit-nbsp-m= ode: space; -webkit-line-break: after-white-space;"><blockquote cite=3D"29C= 78FB6-68C5-425F-9729-ABA4EEDC204D@dal.ca" type=3D"cite" class=3D"cite2"> Hi Dave, <div>I=E2=80=99d forgotten about this, but isn=E2=80=99t the primary observ= ation to explain that the plants routinely sink in the winter and rise agai= n in the spring? =C2=A0What the mechanism is, is an interesting but seconda= ry consideration. =C2=A0 In your =E2=80=98experimental=E2=80=99 case with lighted duckweed, it should be producing and accumulating some sort of gas = mixture by September's photosynthesis, maybe plus some concurrent respirat= ion. =C2=A0Your =E2=80=98control', dark duckweed, should have no photosythe= sic activity, so only respiration should produce any gas at all, presumably CO2.</div> <div>=C2=A0=C2=A0</div> <div>The problem is that neither =E2=80=98expt' nor =E2=80=98control' actua= lly sank on Sept 27. =C2=A0Since the plant material including any dense sta= rch should anyway have an average density >1g/cc (i.e. greater than plai= n water*), excluding any gas spaces, both cases must still be floating according to some undissolved gas inclusions, though not neces= sarily the same mix of CO2/O2 in the two cases. =C2=A0You get an A+ for ind= ustry and design, but <i>Lemna</i> gets an F for cooperation.=C2=A0</div> <div><br /> </div> <div>*Something I=E2=80=99d not considered that you may know about but I do= n=E2=80=99t is oil/fat production in plants, which obviously is important f= or some land plants (canola, sesame,=C2=A0olives=C2=A0etc). =C2=A0Oils have specific gravities <1, around 0.9 g/cc, so enough oil volume could = be sufficient to float a water plant without invoking gas spaces. =C2=A0If = something dense like starch gets converted to an oil in spring, could that = contribute, or is this just fantasy? =C2=A0</div> <div><br /> </div> <div>Is it possible that the trigger for sinking, whatever its mechanism, i= s a particular drop in day length that had not yet been closely approached= by Sept 27, and especially in your toasty living room (temperature might be = another factor)? =C2=A0 If you haven=E2=80=99t flushed them in frustration, you should convey both your test subjects to= an unheated outhouse and extend your experiment there at least to freeze-up= .=C2=A0</div> <div>Steve =C2=A0</div> <div>---------------------------------------------------------<br /> <div> <div>On Sep 27, 2018, at 12:35 PM, David <<a href=3D"mailto:dwebster@gli= nx.com">dwebster@glinx.com</a>> wrote:</div> <br class=3D"Apple-interchange-newline" /> <blockquote type=3D"cite" class=3D"cite"><style type=3D"text/css"><!--#xc5d= d5f1fc8d549c blockquote.cite{ margin-left:5px; margin-right:0px; padding-left:10px; padding-right:0px; border-left:1px solid #CCC ; } #xc5dd5f1fc8d549c blockquote.cite2{ margin-left:5px; margin-right:0px; padding-left:10px; padding-right:0px; border-left:1px solid #CCC; margin-top:3px; padding-top:0px; } #xc5dd5f1fc8d549c a img{ border:0px; } #xc5dd5f1fc8d549c li#xc5dd5f1fc8d549c [style=3D"'text-align: center;'"],#xc= 5dd5f1fc8d549c li#xc5dd5f1fc8d549c [style=3D"'text-align: right;'"]{ list-style-position:inside; } #xc5dd5f1fc8d549c{ font-family:Segoe UI; font-size:12pt; }--></style> <div> <div>Dear All,</div> <div>=C2=A0 =C2=A0 I have had my eye open this summer for some Lemna to tes= t the gas composition idea; the more soluble CO2 predominating as days shor= ten leading to flooding of gas spaces and sinking; photosynthesis predomina= ting in spring as days lengthen leading to flooded air spaces being filled by less soluble O2 and rise to the surface= .=C2=A0</div> <div>=C2=A0=C2=A0=C2=A0=C2=A0And I was able to collect some on Sept 21 with= out having to wade in muck of uncertain depth. So I set up a simple trial;= some kept in darkness, except for a few minutes daily for observation, and= some kept on a windowsill.=C2=A0</div> <div>=C2=A0 =C2=A0 By Sept 27 those kept in darkness showed no tendency to= sink but were noticeably less green than those exposed to daylight and some = artificial light in the evening. This leads to the conclusion that floodin= g of intercellular gas spaces when CO2 predominates does not cause sinking of Lemna. =C2=A0The converse explanation that Lemna = rises to the surface in the spring when intercellular spaces become filled = by less soluble O2 is accordingly voided.</div> <div>=C2=A0 =C2=A0 The explanation given in the link posted by Nancy on Oct = 3, 2017 is consequently more sound. Starch accumulation in the fall. as ph= otosynthesis exceeds respiration at lowering temperature, leads to sinking. = Starch consumption, as temperature warm in the spring and new buds start to grow, leads to rise to the water surface. = [density of starch is high; 1.5 g/cm^3]</div> <div>=C2=A0 =C2=A0=C2=A0</div> <div>Yt, DW, Kentville</div> <div><br /> </div> <div>=C2=A0 =C2=A0=C2=A0</div> <div><br /> </div> <div>------ Original Message ------</div> <div>From: "Stephen Shaw" <<a href=3D"mailto:srshaw@Dal.Ca">srshaw@Dal.C= a</a>></div> <div>To: "<a href=3D"mailto:naturens@chebucto.ns.ca">naturens@chebucto.ns.c= a</a>" <<a href=3D"mailto:naturens@chebucto.ns.ca">naturens@chebucto.ns.= ca</a>></div> <div>Sent: 3/10/2018 3:20:47 PM</div> <div>Subject: Re: [NatureNS] Duckweed</div> <div><br /> </div> <div id=3D"x20c912178f194f2" style=3D"word-wrap: break-word; -webkit-nbsp-m= ode: space; -webkit-line-break: after-white-space;"> <blockquote cite=3D"x-msg://4/F3FE0AE5-B951-4A4A-9575-4CE98894C7CA@dal.ca"= type=3D"cite" class=3D"cite2"> Interesting puzzle, perhaps not so simple. =C2=A0Any cell system like a liv= e leaf is bound to be somewhat denser than pond water, regardless of denser = starch, so will naturally sink at all times unless kept buoyant by the ext= racellular gas bubbles or some other modification. =C2=A0What seems to need explaining is why the bubbles are m= aintained in summer (dissolved gases can pass through lipid cell membranes= quickly, so you=E2=80=99d expect them to dissolve out into the surrounding= pond), and why they disappear in winter. =C2=A0He doesn=E2=80=99t seem to know if it=E2=80=99s CO2 or O2 in the bubbles, or= both. =C2=A0Perhaps they are largely O2 in summer and dissolve and normally = pass out of the leaf to oxygenate the pond and dissolved CO2 moves in, but = leaf photosynthesis is sufficiently high in summer to maintain them, despite these losses. =C2=A0In winter gas production simply = stops, and the non-buoyant plants sink. =C2=A0As winter ends, respiration= produces bubbles with a different gas, CO2, and they rise again then switch = over to O2 as photosynthesis picks up. =C2=A0=C2=A0 <div><br /> </div> <div>It therefore seems likely that these leaves have some special surface= coating that slows down gas exchange with the pond. =C2=A0Maybe they still= retain stomata under the leaf which normally facilitate gas exchange for le= aves in air, but these are modified to block or regulate gas exchange in water? =C2=A0Maybe this is already known= , just not to us here?</div> <div>Steve =C2=A0=C2=A0<br /> <br /> <div> <div>On Mar 10, 2018, at 10:45 AM, David <<a href=3D"mailto:dwebster@gli= nx.com">dwebster@glinx.com</a>> wrote:</div> <br class=3D"Apple-interchange-newline" /> <blockquote type=3D"cite" class=3D"cite"> <div style=3D"font-family: Tahoma; font-size: 12pt; font-style: normal; fon= t-variant: normal; font-weight: normal; letter-spacing: normal; line-height= : normal; orphans: auto; text-align: start; text-indent: 0px; text-transfor= m: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text= -stroke-width: 0px;"> <div>Hi Nancy & All,</div> <div>=C2=A0 =C2=A0 I admit not having wondered about this but I think he ma= kes a simple process complicated. The air pockets to which he refers are pr= esumably intercellular spaces which in the absence of photosynthesis presum= ably can become water filled. Starch has a specific gravity of 1.5 g/mL and, in cooler weather starch will accumulate = when respiration slows more than photosynthesis and the submarine will sin= k. With warming, growth resumes, respiration and starch consumption rates i= ncrease and =C2=A0the submarine rises.</div> <div>Yt, DW, Kentville</div> <div><br /> </div> <div>------ Original Message ------</div> <div>From: "nancy dowd" <<a href=3D"mailto:nancypdowd@gmail.com">nancypd= owd@gmail.com</a>></div> <div>To:<span class=3D"Apple-converted-space">=C2=A0</span><a href=3D"mailt= o:naturens@chebucto.ns.ca">naturens@chebucto.ns.ca</a></div> <div>Sent: 3/10/2018 9:08:02 AM</div> <div>Subject: [NatureNS] Duckweed</div> <div><br /> </div> <div id=3D"xd49ec0a2bdf4406"> <blockquote cite=3D"x-msg://1/A593EEEC-616D-49BA-B70F-CA21003EAEDB@gmail.co= m" type=3D"cite" class=3D"cite2" style=3D"margin-left: 5px; margin-right: 0= px; padding-left: 10px; padding-right: 0px; border-left-width: 1px; border-= left-style: solid; border-left-color: rgb(204, 204, 204); margin-top: 3px;= padding-top: 0px;"> This is an interesting article on Duckweed seasonal disappearance and reapp= earance in the Spring 2018 issue of Northern Woodlands Magazine.<a href=3D"= https://northernwoodlands.org/outside_story/article/duckweed-migration">htt= ps://northernwoodlands.org/outside_story/article/duckweed-migration</a> <div><br /> </div> <div>I had never really thought about where it went in Fall or how it re-em= erges in Spring.=C2=A0<span style=3D"background-color: rgba(255, 255, 255,= 0);">Such an important floating pond plant in productive fresh water=C2=A0<= /span><br /> <div><br /> </div> <div>Nancy D<br /> <br /> <div>Sent from my iPad</div> </div> </div> </blockquote> </div> </div> </blockquote> </div> <br /> </div> </blockquote> </div> </div> </blockquote> </div> <br /> </div> </blockquote></div> </body></html> --------=_MB71654DB8-2E04-493B-B3A4-647F7308BA4F--
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Index of Subjects